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Expression of the S ‐locus receptor kinase multigene family in Brassica oleracea
Author(s) -
Kumar Vinod,
Trick Martin
Publication year - 1994
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1994.6060807.x
Subject(s) - brassica oleracea , biology , gene , locus (genetics) , genetics , complementary dna , microbiology and biotechnology , intron , restriction fragment length polymorphism , gene family , genomic dna , gene expression , polymerase chain reaction , botany
Summary The S ‐receptor kinase ( SRK ) gene which is implicated in the self‐incompatibility system of Brassica oleracea is one member of a large and complex family of similar sequences. Genomic and cDNA clones were isolated for the authentic, S ‐linked SRK 29 gene and its DNA sequence determined. Reverse transcriptase PCR (RT‐PCR) was used to detect the expression of SRK,29 and other members of the family in stigma, leaf and root tissues. The SRK was found to be stigma‐specific whereas, for instance, K 3 transcripts appeared in all three tissues. The RT‐PCR analysis also demonstrated the existence of partially processed intermediates for several of the kinase transcripts and, in the case of SRK 29 , a product apparently resulting from the splicing of a cryptic intron. RFLP analysis of an F 2 family segregating for the S 29 allele was used to show S ‐linkage for the SRK and possibly for the K 2 sequence. The K 8 kinase probe also revealed a minor RFLP which segregated with the S ‐locus.