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Circadian clock‐ and phytochrome‐regulated transcription is conferred by a 78 bp cis ‐acting domain of the Arabidopsis CAB2 promoter
Author(s) -
Anderson Shawn L,
Teakle Graham R,
MartinoCatt Susan J,
Kay Steve A
Publication year - 1994
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1994.6040457.x
Subject(s) - arabidopsis , phytochrome , circadian clock , biology , promoter , luciferase , transcription factor , mutant , microbiology and biotechnology , circadian rhythm , transcription (linguistics) , dna binding protein , genetics , dna binding domain , gene , gene expression , transfection , botany , linguistics , red light , philosophy , neuroscience
The Arabidopsis CAB2 promoter was used to define the terminal genomic targets that are subject to regulation by the circadian clock. An in vivo cab::luciferase bioluminescent marker was used to enable the assaying of the expression of chimeric constructs with unprecedented sensitivity and time resolution in living seedlings. Dissection of -322 to +1 of the CAB2 promoter has revealed several interesting features: it was demonstrated that the 323 bp fragment contains at least one strong general positive element. The positive element contains an ACGT core sequence specifically bound by a protein activity, termed CUF-1, and contributes to high level expression but is not required for phytochrome- or circadian-regulation. Moreover, a 78 bp domain was defined that confers both circadian- and phytochrome-regulation upon heterologous promoters. Conserved GATA sequences within the 78 bp regulatory domain are specifically bound by a protein factor designated CGF-1. The binding specificity of CGF-1 appears to be related to the GT-family of trihelix DNA-binding proteins. The role of these DNA-protein interactions is discussed in terms of clock- and phytochrome regulation, and their relevance as targets for pathways defined by photomorphogenic mutants.