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Genetic control of dihydroflavonol 4‐reductase gene expression in Petunia hybrida
Author(s) -
Huits Henk S.M.,
Gerats Anton G.M.,
Kreike Marja M.,
Mol Joseph N.M.,
Koes Ronald E.
Publication year - 1994
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1994.06030295.x
Subject(s) - biology , gene , locus (genetics) , genetics , complementation , petunia , transgene , stamen , ectopic expression , botany , phenotype , pollen
The functions of four loci ( An1, An2, An4 , and An6 ) which control pigmentation in flowers of Petunia hybrida have been characterized. Linkage‐analysis and molecular complementation experiments showed that the An6 locus contains the structural dfrA gene, encoding the enzyme dihydroflavonol 4‐reductase (DFR). Analysis of gus gene expression driven by the dfrA promoter in transgenic plants showed that the dfrA promoter is highly active in the flower corolla, the anthers and seeds and, at a lower level, in ovules and the flower stem. These data are discussed in relation to the expression of other pigmentation genes and the accumulation pattern of anthocyanins. The expression of the dfrA‐gus transgene was dependent on the genes an1 (in every tissue), an2 (in the flower limb only) and an4 (in anthers), demonstrating that these genes encode regulatory factors that control dfrA promoter activity.