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A new class of tobacco chitinases homologous to bacterial exo‐chitinases displays antifungal activity
Author(s) -
Melchers Leo S.,
Groot Marion Apothekerde,
Knaap Jan A.,
Ponstein Anne S.,
SelaBuurlage Marianne B.,
Bol John F.,
Cornelissen Ben J.C.,
Elzen Peter J.M.,
Linthorst Huub J.M.
Publication year - 1994
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1994.05040469.x
Subject(s) - chitinase , antifungal , homologous chromosome , microbiology and biotechnology , class (philosophy) , biology , chemistry , biochemistry , enzyme , computer science , gene , artificial intelligence
Summary A novel chitinase gene of tobacco was isolated and characterized by DNA sequence analysis of a genomic clone and a cDNA clone. Comparative sequence analysis of both clones showed an identity of 94%. The proteins encoded by these sequences do not correspond to any of the previously characterized plant chitinases of classes I–IV and are designated as class V chitinases. Comparison of the chitinase class V peptide sequence with sequences in the Swiss Protein databank revealed significant sequence similarity with bacterial exo‐chitinases from Bacillus circulans , Serratia marcescens and Streptomyces plicatus . It was demonstrated that class V chitinase gene expression is induced after treatment of tobacco with different forms of stress, like TMV‐infection, ethylene treatment, wounding or ultraviolet irradiation. Two related chitinase class V proteins of 41 and 43 kDa were purified from Samsun NN tobacco leaves inoculated with tobacco mosaic virus. The proteins were purified by Chelating Superose chromatography and gel filtration. In vitro assays demonstrated that class V chitinases have endo‐chitinase activity and exhibit antifungal activity toward Trichoderma viride and Alternaria radicina . In addition, it was shown that class V chitinase acts synergistically with tobacco class I β‐1,3‐glucanase against Fusarium solani germlings.