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FIL2, an extracellular Leucine‐Rich Repeat protein, is specifically expressed in Antirrhinum flowers
Author(s) -
Steinmayr Markus,
Motte Patrick,
Sommer Hans,
Saedler Heinz,
SchwarzSommer Zsuzsanna
Publication year - 1994
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1994.05040459.x
Subject(s) - antirrhinum , biology , leucine rich repeat , genetics , gene , homeotic gene , petal , microbiology and biotechnology , mutant , botany , arabidopsis
Summary The expression of the Antirrhinum gene FIL2 is affected in mutants of the homeotic transcription factor DEFICIENS . Northern and Western blot analyses showed that FIL2 in wild‐type Antirrhinum flowers is expressed weakly in the petals and more abundantly in the reproductive organs; the gene is active in the filaments and anthers of stamens, and in the stigma and transmitting tissue of the carpels. The FIL2 protein is glycosylated with high mannose type glycan chains and is located in the middle lamella of the extracellular matrix. The amino acid sequence contains 10 tandem repeats, the composition of which is similar to the L eucine‐ R ich R epeat (LRR) motif found in mammals, Drosophila and yeast. The possiblity that FIL2 might be a component of a cellular signalling mechanism, involving LRR‐mediated protein‐protein interactions is discussed.