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A non‐specific lipid transfer protein from Arabidopsis is a cell wall protein
Author(s) -
Thoma Sharon,
Kaneko Yasuko,
Somerville Chris
Publication year - 1993
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1993.t01-25-00999.x
Subject(s) - arabidopsis , plant lipid transfer proteins , immunoelectron microscopy , microbiology and biotechnology , intracellular , phospholipid transfer protein , fusion protein , biology , arabidopsis thaliana , chemistry , biochemistry , membrane , antibody , phospholipid , gene , genetics , mutant , recombinant dna
Summary Lipid transfer proteins (LTPs), mediate the transfer of phospholipids between membranes in vitro. However, the in vivo function of LTPs is not known. To determine the precise location of a non‐specific LTP from Arabidopsis , a cDNA clone was used to produce an Arabidopsis LTP:protein A fusion. Antibodies raised against the fusion were used to localize the Arabidopsis LTP by immunoelectron microscopy. LTP was found to be located in the cell wall, mainly in epidermal cells. This location appears to be inconsistent with the proposed role of the protein in intracellular lipid transfer.