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Light‐regulated and endogenous fluctuations of chloroplast transcript levels in Chlamydomonas . Regulation by transcription and RNA degradation
Author(s) -
Salvador Maria L.,
Klein Uwe,
Bogorad Lawrence
Publication year - 1993
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1993.t01-13-00999.x
Subject(s) - chlamydomonas reinhardtii , biology , chloroplast , transcription (linguistics) , plastid , gene , gene expression , organelle , chlamydomonas , microbiology and biotechnology , rna , endogeny , genetics , mutant , biochemistry , linguistics , philosophy
Summary Changes in the relative sizes of pools of transcripts of organelle genes during plastid development are common in flowering plants, but technical difficulties have prevented direct determinations of the effects of changes in rates of transcription and degradation on such fluctuations. It has been possible to follow both rates in Chlamydomonas reinhardtii . In synchronous or asynchronous cultures of cells grown in 12 h light/12 h dark periods, sizes of pools of transcripts of the chloroplast genes atp A, atp B, tuf A, and psa B fluctuate. Differences in chloroplast transcript abundances in light/dark cycles were found to be cell cycle‐independent but controlled by either an endogenous rhythm ( atp A, atp B, and tuf A) or by light ( psa B). In vivo labeling experiments showed that transcriptional regulation and light/dark‐regulated degradation both contribute, in gene‐specific manners, to the level of transcripts of individual C. reinhardtii chloroplast genes in cells grown in alternating light/dark cycles.