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Detection of point mutations in the chloroplast genome by single‐stranded conformation polymorphism analysis
Author(s) -
To KinYing,
Liu ChiuI.,
Liu ShihTung,
Chang YuSun
Publication year - 1993
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1993.t01-10-00999.x
Subject(s) - microbiology and biotechnology , single strand conformation polymorphism , biology , point mutation , dna , genetics , polymerase chain reaction , mutant , chloroplast dna , locus (genetics) , genome , gene
Summary Single‐stranded conformation polymorphism (SSCP) analysis was used to examine the mutations of the chloroplast 16S rRNA locus of streptomycin‐resistant mutants in Nicotiana plumbaginifolia. DNA fragments of 121, 517, 968 and 1578 bp, each possessing a known point mutation, were generated by polymerase chain reaction (PCR). The resulting fragments were denatured and separated by nondenaturing polyacrylamide gel electrophoresis. Compared to the patterns of the wild‐type DNA fragments, the bands of the single‐stranded DNA fragments of 121 and 517 bp with base changes were shifted. However, no pattern variations were detected from the DNA fragments of 968 and 1578 bp generated from both wild‐type and mutants.