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An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana †
Author(s) -
Höfte Herman,
Desprez Thierry,
Amselem Joëlle,
Chiapello Hélène,
Caboche Michel,
Moisan Annick,
Jourjon MarieFrançoise,
Charpenteau JeanLouis,
Berthomieu Pierre,
Guerrier Danièle,
Giraudat Jérome,
Quigley Françoise,
Thomas Frank,
Yu DeYao,
Mache Régis,
Raynal Monique,
Cooke Richard,
Grellet Françoise,
Delseny Michel,
Parmentier Yves,
Marcillac Guy,
Gigot Claude,
Fleck Jacqueline,
Philipps Gabriel,
Axelos Michèle,
Bardet Claude,
Tremousaygue Dominique,
Lescure Bernard
Publication year - 1993
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1993.04061051.x
Subject(s) - arabidopsis thaliana , sequence (biology) , biology , computational biology , arabidopsis , genetics , gene , mutant
As part of the goal to generate a detailed transcript map for Arabidopsis thaliana , 1152 single run sequences (expressed sequence tags or ESTs) have been determined from cDNA clones taken at random in libraries prepared from different sources of plant material: developing siliques, etiolated seedlings, flower buds, and cultured cells. Eight hundred and ninety‐five different genes could be identified, 32% of which showed significant similarity to existing sequences in Arabidopsis and an array of other organisms. These sequences in combination with their positioning on the Arabidopsis genetic map will not only constitute a new set of molecular markers for genome analysis in Arabidopsis but also provide a direct route for the in vivo analysis of their gene products. The sequences have been made available to the public databases.