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The anther‐specific protein encoded by the Brassica napus and Arabidopsis thaliana A6 gene displays similarity to β‐1,3‐glucanases
Author(s) -
Hird Diane L.,
Worrall Dawn,
Hodge Rachel,
Smartt Sarah,
Paul Wyatt,
Scott Rod
Publication year - 1993
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1993.04061023.x
Subject(s) - brassica , arabidopsis thaliana , gene , arabidopsis , similarity (geometry) , genetics , stamen , biology , computational biology , botany , computer science , artificial intelligence , mutant , pollen , image (mathematics)
An anther‐specific Brassica napus cDNA, A6, and two corresponding Arabidopsis thaliana genes have been isolated. Sequence analyses of A6 revealed similarity to β‐1,3‐glucanases. The deduced A6 protein differs from other β‐1,3‐glucanases in the possession of a long C‐terminus. Immunoblotting using an antibody raised to the A6 protein detects a temporal 60 kDa protein in B. napus buds, suggesting that the long C‐terminal region is present in the mature protein. A6 promoter—GUS and RNase fusions demonstrate that the A6 gene is tapetum‐specific and temporally expressed with a peak in activity when the plant normally expresses callase (a complex of endo‐ and exo‐β‐1,3‐glucanase activities). The sequence similarity of A6 to other β‐1,3‐glucanases, coupled with the temporal and spatial expression data, suggests that A6 may be part of the callase enzyme complex.