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PCR amplification of microdissected wheat chromosome arms in a simple ‘single tube’ reaction
Author(s) -
Albani Diego,
Côté MarieJosé,
Armstrong Ken C.,
Chen Qianfa,
Segal Allan,
Robert Laurian S.
Publication year - 1993
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1993.04050899.x
Subject(s) - chromosome , tube (container) , biology , microbiology and biotechnology , polymerase chain reaction , genetics , chemistry , materials science , gene , composite material
A novel method for the adaptor‐mediated PCR amplification of microdissected chromosome arms is described. This simple and versatile protocol eliminates the need for enzymatic micromanipulation in nanoliter volumes and permits the efficient amplification of as little as two wheat chromosome arms in a ‘single tube’ reaction.