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Recombinant inbred lines for mapping RFLP and phenotypic markers in Arabidopsis thaliana
Author(s) -
Lister Clare,
Dean Caroline
Publication year - 1993
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1993.04040745.x
Subject(s) - inbred strain , restriction fragment length polymorphism , biology , arabidopsis thaliana , ecotype , genetics , arabidopsis , gene mapping , phenotype , recombinant dna , genetic marker , mutant , genotype , gene , chromosome
Recombinant inbred (RI) lines of Arabidopsis thaliana (Arabidopsis) have been generated from a cross between the ecotypes Landsberg erecta and Columbia. Progeny of 300 individual F 2 seedlings were taken by single seed descent to the F 8 generation. Sixty‐seven loci, scored using 64 RFLP probes and one phenotypic marker, chosen at approximately 20 cM intervals from the two previously published RFLP maps, were mapped using 100 of these RI lines. More than 500 other new loci are currently being mapped using these RI lines by several other groups. These 100 RI lines thus provide the material to map new probes or phenotypic traits polymorphic between Landsberg erecta and Columbia, relative to an increasing number of molecular markers. Higher resolution mapping of distinct chromosomal regions can be achieved by analysing the segregation of particular markers on the additional 200 RI lines.