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Analysis of large DMA from soybean (Glycine max L. Merr.) by pulsed‐field gel electrophoresis
Author(s) -
Honeycutt Rhonda J.,
Sobral Bruno W.S.,
McClelland Michael,
Atherly Alan G.
Publication year - 1992
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1992.t01-53-00999.x
Subject(s) - pulsed field gel electrophoresis , gel electrophoresis , biology , genome , glycine , electrophoresis , isolation (microbiology) , physical mapping , genetics , microbiology and biotechnology , gene mapping , gene , chromosome , genotype , bioinformatics , amino acid
Summary The technique of pulsed‐field gel electrophoresis (PFE) has been used to study chromosomal regions and entire genomes of several organisms. Techniques are presented for the isolation of high molecular weight DMA from embedded soybean protoplasts and the conditions for separating large DMA fragments using PFE. Digestion was detected by Southern hybridization using single copy nodulin clones. These data are being used to generate a physical map of the nodulin region(s) of the soybean genome.