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Stringent repression and homogeneous de‐repression by tetracycline of a modified CaMV 35S promoter in intact transgenic tobacco plants
Author(s) -
Gatz Christiane,
Frohberg Claus,
Wendenburg Regina
Publication year - 1992
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1992.t01-37-00999.x
Subject(s) - cauliflower mosaic virus , derepression , transgene , psychological repression , repressor , inducer , genetically modified crops , biology , transformation (genetics) , reporter gene , microbiology and biotechnology , tetracycline , genetics , gene , gene expression , antibiotics
Summary A cauliflower mosaic virus (CaMV) 35S promoter derivative, which is tightly repressed by the Tn 10 encoded Tet repressor in a transient expression system as well as in transgenic plants has been constructed. After treatment of transgenic plants with tetracycline (Tc) the activity of the reporter enzyme β‐glucuronidase (GUS) increased up to 500‐fold in tissue culture as well as under greenhouse conditions. Efficient derepression was achieved by Tc uptake through the roots as well as by Tc treatment of leaves of intact plants. As Tc is not very stable in the plants, this system can also be used for a transient expression of a transgene. This system provides a unique tool for regenerating transgenic plants carrying a repressed transgene and for efficiently de‐repressing its activity by a specific inducer at any time point of further development.