Premium
Metabolic regulation of rice α‐amylase and sucrose synthase genes in planta
Author(s) -
Karrer Erik E.,
Rodriguez Raymond L.
Publication year - 1992
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1992.t01-22-00999.x
Subject(s) - endosperm , sucrose synthase , scutellum , sucrose , gene , isozyme , gene expression , biochemistry , biology , oryza sativa , embryo , enzyme , atp synthase , amylase , invertase , microbiology and biotechnology
Summary Isolated rice embryos were used to investigate the regulatory effects of endosperm extracts and pure sugars on the expression of α‐amylase gene RAmy3D and a sucrose synthase gene homologous to the maize isozyme Ss2 . The high‐level expression of RAmy3D in the scutella of isolated embryos could be inhibited by a variety of sugars as well as endosperm extracts from germinated rice grains. Glucose, at a concentration of 250 mM, was most effective in repressing RAmy3D mRNA accumulation. Furthermore, this repression was reversible. Interestingly, RAmy3D repression was always accompanied by the induction of sucrose synthase gene expression. These results support a model in which the expression of α‐amylase and sucrose synthase genes in the rice scutellum are counter‐regulated by the influx of sugars from the endosperm.