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The promoter of the rice gene GOS2 is active in various different monocot tissues and binds rice nuclear factor ASF‐1
Author(s) -
Pater B. Sylvia,
Mark Frits,
Rueb Saskia,
Katagiri Fumiaki,
Chua NamHai,
Schilperoort Rob A.,
Hensgens Lambert A. M.
Publication year - 1992
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1992.00999.x
Subject(s) - gene , promoter , biology , reporter gene , transcription factor , oryza sativa , transcription (linguistics) , microbiology and biotechnology , genetics , dna , gene expression , linguistics , philosophy
Summary A single copy gene has been isolated, termed GOS2 , from rice. Sequence comparison revealed highly similar genes in mammals and yeast, indicating that GOS2 encodes an evolutionary conserved protein. GOS2 mRNA was detected in all tissues examined. When the upstream region was translationally fused to the reporter gene gusA it was found to drive expression in a variety of rice tissues and in cell suspensions of other monocot species following introduction by particle bombardment. Therefore, the GOS2 promoter is potentially useful for genetic engineering of monocots. A DNA‐binding activity from rice, termed rice ASF‐1, with similar binding specificity as the cloned tobacco transcription factor TGA‐1a, was found to bind to a TGACG sequence motif in the GOS2 promoter. Possible roles for rice ASF‐1 in the transcriptional activation of the GOS2 promoter are discussed.