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Identification and molecular mapping of a single Arabidopsis thaliana locus determining resistance to a phytopathogenic Pseudomonas syringae isolate
Author(s) -
Debener Thomas,
Lehnackers Hiltrud,
Arnold Martin,
Dangl Jeffery L
Publication year - 1991
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1991.t01-7-00999.x
Subject(s) - pseudomonas syringae , pathovar , biology , pathosystem , genetics , locus (genetics) , gene , arabidopsis , arabidopsis thaliana , plant disease resistance , gene mapping , pseudomonas , pseudomonadaceae , chromosome , bacteria , mutant
Summary We present a model pathosystem to dissect genetically the disease resistance response of plants against phytopathogenic bacteria. The interaction between Pseudomonas syringae pathovar maculicola (Psm) and Arabidopsis thaliana displays phenotypic variaion which depends on the genotype of both partners. Compatible interactions are defined by sustained inplanta bacterial growth and are normally accompanied of their appearance. For compatible interactions, resistance is defined by limited in‐planta bacterial growth accompanied by a typical ‘hypersensitive response’ (HR). We show that at least parts of this system fit the paradigms of Flor’s ‘gene‐for‐gene’ hypothesis. We identify functionally a putative bacterial avirulence gene (avrRpm 1) from a Psm isolate which conditions the HR on A. thaliana ecotypes Oy‐0 abd Col‐ 0, but not Nd‐0. We also demonstrate that resistance to the Psm strain from which avrRpm1 was isolated segregates as a single trait in the crosses Col‐o x Nd‐0 and Nd‐0 x Oy‐0. Furthermore, we map this locus (RPM1) molecularly in the Col‐0 x Nd‐0 cross to a relatively small interval defined by two RFLP markers on A. thliana chromosome 3. Resistance in the second cross also maps to this locus and cosegregates with resistance to avrRpm1.