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T‐DNA as a gene tag
Author(s) -
Richard Walden,
Hayashi Hiroaki,
Schell Jeff
Publication year - 1991
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1991.t01-6-00999.x
Subject(s) - biology , genetics , selectable marker , gene , insertional mutagenesis , dna , transformation (genetics) , mutagenesis , agrobacterium , mutant , transgene , computational biology , microbiology and biotechnology
Summary The characteristics of Agrobacterium‐mediated plant transformation are such that the T‐DNA can be used as an insertional mutagen and, by extension, a gene tag. An increasing number of mutants have been obtained as a result of T‐DNA insertion and the versatility of this experimental system can be exploited further to tag sequences of DNA which control gene expression. While the isolation of specific mutations by T‐DNA tagging in the past has been fortuitous, T‐DNA‐based vectors have the potential to generate transgenic plants with specific, selectable mutations.