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Identification and map position of YAC clones comprising one‐third of the Arabidopsis genome.
Author(s) -
Hwang I,
Kohchi T,
Hauge BM,
Goodman HM,
Schmidt R,
Cnops G,
Dean C,
Gibson S,
Iba K,
Lemieux B
Publication year - 1991
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.1991.t01-5-00999.x
Subject(s) - arabidopsis , genome , biology , genetics , arabidopsis thaliana , insert (composites) , restriction fragment length polymorphism , gene mapping , clone (java method) , identification (biology) , computational biology , gene , chromosome , botany , genotype , mutant , mechanical engineering , engineering
Summary YAC clones corresponding to 125 Arabidopsis thaliana RFLP markers have been identified. At least one YAC clone has been isolated for each of the RFLP markers tested. Based on CHEF gel analysis of 196 clones, the mean insert size of the available Arabidopsis YAC libraries is approximately 160 kb. The YACs of known genetic map location encompass about 30% of the Arabidopsis genome. The results presented here represent a first step towards assembly of an overlapping YAC library of the A. thaliana genome.