Premium
Temperature Alters Lipopolysaccharide‐Induced Cytokine Secretion by RAW 264.7 Cells
Author(s) -
Kirkley J. E.,
Thompson B. J.,
Coon J. S.
Publication year - 2003
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2003.01274.x
Subject(s) - secretion , lipopolysaccharide , cytokine , tumor necrosis factor alpha , incubation , interleukin , chemistry , medicine , endocrinology , interleukin 6 , immune system , biology , immunology , biochemistry
Local and systemic temperature change is associated with the immune response to infection, but the role of temperature remains poorly understood. To study the effect of temperature on macrophage activation by lipopolysaccharide (LPS), RAW 264.7 cells were incubated with LPS at different temperatures and secretion of three cytokines was measured. Incubation at 31 °C increased tumour necrosis factor (TNF) secretion when compared with 37 °C, while cells exposed at 39 °C secreted less TNF. Interleukin‐6 (IL‐6) secretion was less at 31 °C than at 37 °C and remained unchanged at 39 °C. Interleukin‐10 secretion was depressed on either side of 37 °C. Only IL‐6 secretion was sensitive to preincubation temperature effects. The kinetics of cytokine secretion and steady‐state mRNA analysis indicated potentially different mechanisms of temperature regulation for TNF and IL‐6.