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Monocyte‐Derived CD1a + Dendritic Cells Generated in Two Different Culture Systems: Immunophenotypic and Functional Comparison
Author(s) -
Xia C.Q.,
Kao K. J.
Publication year - 2003
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2003.01238.x
Subject(s) - cd86 , cd80 , dendritic cell , cd40 , priming (agriculture) , cd11c , follicular dendritic cells , microbiology and biotechnology , immunology , biology , t cell , antigen presenting cell , chemistry , immune system , in vitro , phenotype , cytotoxic t cell , biochemistry , botany , germination , gene
Previous studies demonstrated that CD1a + dendritic cells (DCs) could not be prepared ex vivo without using fetal calf serum (FCS). Recently, we developed a method of using heparin to induce differentiation of human monocytes into CD1a + DCs without using FCS. In order to determine the potential clinical applicability of heparin‐induced CD1a + DCs, we conducted this study to compare both types of CD1a + DCs, immunophenotypically and functionally. Our results showed that the expression of CD1a on heparin‐DCs was lower than that on FCS‐DCs. Both types of DCs expressed similar levels of CD11c, HLA‐DR, CD40, CD83, CD80 and CD86 before and after lipopolysaccharide stimulation. Immature heparin‐DCs and FCS‐DCs had similar phagocytic activities. Heparin‐DCs consistently secreted higher interleukin‐10 (IL‐10) and lesser IL‐12 than FCS‐DCs after activation. Mature heparin‐DCs were slightly more active than mature FCS‐DCs in stimulating the proliferation of allogeneic CD4 + T cells. Both types of mature CD1a + DCs primed the naïve CD4 + T cells to produce large amount of interferon‐γ (IFN‐γ). However, naïve CD4 + T cells stimulated with FCS‐DCs produced more IFN‐γ, while the naïve CD4 + T cells stimulated with heparin‐DCs produced more IL‐5. The results indicate that both types of CD1a + DCs do not have identical function in the priming of CD4 + T cells and have minor difference in immunophenotypes.