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Immunoglobulin G3 and Immunoglobulin M Isotype Plasma Levels are Influenced by Interleukin‐1α Genotype
Author(s) -
Kilpinen S.,
Laine S.,
Hulkkonen J.,
Hurme M.
Publication year - 2003
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2003.01231.x
Subject(s) - genotype , antibody , isotype , biology , immunology , immune system , immunoglobulin g , interleukin 4 , gene , genetics , monoclonal antibody
The immunoglobulin (Ig) plasma levels are known to be, at least partially, genetically regulated, but all the genes involved are not known. Interleukin‐1 (IL‐1) is a potent proinflammatory cytokine able to serve as an adjuvant for immune responses. IL‐1α gene is polymorphic, and at least one of the polymorphisms has been identified in the 5′ regulatory region of the promoter, a biallelic base exchange (C→T) at position −889. We set out to study whether the IL‐1α genotype might contribute to the genetic component seen in the steady‐state antibody levels of healthy individuals. Four hundred healthy blood donors (218 males and 182 females) were genotyped, and the plasma levels of IgM, IgG as well as IgG subclasses were measured. An association was found between IgG3 plasma levels and the IL‐1α genotype; the 1.1 homozygotes had increased IgG3 levels compared with the 1.2 heterozygotes ( P < 0.001 in males and P = 0.04 in females, Mann–Whitney U ‐test). A similar significant association was also found between IgM plasma levels and the IL‐1α genotype in males, but it was no longer present in females; the 1.1 homozygotes had higher IgM levels than the 2.2 homozygotes ( P = 0.03, Mann–Whitney U ‐test). The data suggest that IL‐1α‐mediated signals are critical for IgG3 and IgM responses, which are induced by thymus‐independent antigens and are important in activating complement.