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Comparison of Immunoglobulin Heavy Chain Rearrangements Between Peripheral and Glandular B Cells in a Patient with Primary Sjögren's Syndrome
Author(s) -
Hansen A.,
Jacobi A.,
Pruss A.,
Kaufmann O.,
Scholze J.,
Lipsky P. E.,
Dörner T.
Publication year - 2003
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2003.01226.x
Subject(s) - sialadenitis , polyclonal antibodies , antibody , population , immunoglobulin light chain , myoepithelial cell , cd19 , gene rearrangement , pathology , parotid gland , biology , peripheral blood , microbiology and biotechnology , immunology , salivary gland , medicine , immunohistochemistry , gene , genetics , environmental health
Myoepithelial sialadenitis (MESA) of the major salivary glands is a characteristic feature of primary Sjögren's syndrome (pSS). To delineate systemic and organ‐specific influences on B cells in a patient with pSS and benign MESA, individual B cells were simultaneously obtained from the peripheral blood and inflamed parotid gland. Immunoglobulin variable heavy chain (V H ) rearrangements in single sorted CD19 + B cells were subsequently amplified, sequenced and analysed. Despite the presence of two clonal expansions using V H 1‐08 and V H 2‐70 segments, respectively, the majority of glandular B cells were polyclonal, resembling the V H gene usage and mutational pattern of the corresponding blood population. However, striking differences were observed in the proportion of cells expressing mutated V H rearrangements (blood, 28.9% versus parotid, 80.4%; P < 0.0001). Moreover, the glandular productive V H rearrangements differed significantly from their blood counterparts by a higher mutational frequency ( P < 0.0001), shorter CDR3 lengths ( P = 0.001) and a less frequent usage of J H 6 ( P = 0.0292), indicating an accumulation of memory B cells in the inflamed parotid. Thus, both preferential influx/homing of memory B cells and local proliferation may contribute to the pattern of benign MESA in pSS. Notably, one of the glandular clonal rearrangements (using V H 1‐08) was also detected in the patient's peripheral repertoire.