Premium
Antineutrophil Cytoplasmic Antibodies Induce Decreased CD62L Expression and Enhanced Metabolic Activity in Monocytes
Author(s) -
Wikman A.,
Fagergren A.,
Forslid J.,
Jacobson S. H.,
Johansson S. G. O.,
Lundahl J.
Publication year - 2003
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2003.01209.x
Subject(s) - myeloperoxidase , incubation , chemistry , monocyte , integrin alpha m , monoclonal antibody , hydrogen peroxide , anti neutrophil cytoplasmic antibody , antibody , in vitro , cell adhesion molecule , downregulation and upregulation , immunology , microbiology and biotechnology , l selectin , biochemistry , medicine , inflammation , biology , cell , vasculitis , disease , gene
Monocyte in vitro activation by antimyeloperoxidase (anti‐MPO)‐ and antiproteinase‐3 (anti‐PR3)‐positive sera, corresponding immunoglobulin G (IgG) fractions and monoclonal antibodies against MPO and PR3 was evaluated. The expression of adhesion molecules, l ‐selectin (CD62L) and CR3 (CD11b), involved in leucocyte endothelial adhesion, and metabolic activity, measured as the production of hydrogen peroxide, were analysed. Decreased expression of CD62L was demonstrated in monocytes after incubation with antineutrophil cytoplasmic antibody (ANCA)‐positive sera. This finding was not accompanied by changes in CD11b expression. Metabolic activity was increased in monocytes after incubation with ANCA‐positive IgG fractions as well as after incubation with monoclonal anti‐MPO and anti‐PR3. These findings support the concept that the pathophysiological effect of ANCA is partly mediated through the action on crucial events in monocyte activation, such as CD62L downregulation and oxygen radical production.