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Single‐Cell Analysis of Ets‐1 Transcription Factor Expression during Lymphocyte Activation and Apoptosis
Author(s) -
Koskela K.,
Lassila O.
Publication year - 2003
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2003.01192.x
Subject(s) - transcription factor , apoptosis , microbiology and biotechnology , lymphocyte , expression (computer science) , biology , cell , immunology , genetics , gene , computer science , programming language
The Ets‐1 proto‐oncogene is a prototype member of Ets family of transcription factors. It is preferentially expressed in lymphoid cells, where it is essential for the maintenance of the normal pool of resting T and B cells. We have investigated the protein expression of the Ets‐1 transcription factor during the activation and apoptosis of T and B cells by flow cytometry and confocal microscopy. Cells of the thymus, spleen and bursa expressed high levels of Ets‐1 protein, while resting peripheral blood mononuclear cells had lower Ets‐1 expression. Activation and proliferation of T cells induced the upregulation of Ets‐1. αβ‐T cells were found to upregulate Ets‐1 expression more than γδ‐T cells. Increased Ets‐1 protein expression was located predominantly in the perinuclear area. In contrast, during apoptosis, Ets‐1 expression was downregulated. Collectively, our results indicate that Ets‐1 expression can be accurately determined by flow cytometry and confocal microscopy. Ets‐1 expression level and distribution are differentially controlled in resting, activated and apoptotic lymphocytes.