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Analysis of Complement‐Bound HCV Complexes Using a Novel Immuno‐Capture RT‐PCR Method
Author(s) -
Zhu H.,
Wang S. Y.,
Zhang J. Y.,
Lian Q. Z.,
Peng X. X.
Publication year - 2002
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2002.01164.x
Subject(s) - pathogenesis , antibody , immune system , virology , hepatitis c virus , immunology , polymerase chain reaction , reverse transcriptase , biology , virus , gene , genetics
Recently, more and more evidence has supported the hypothesis that liver cell injury was immune‐mediated in patients with hepatitis C virus (HCV) infection, and that circulating immune complexes (CICs) might play a role in the pathogenesis of chronic hepatitis C (HC). In the present study, we have combined immuno‐capture and reverse transcriptase‐polymerase chain reaction (RT‐PCR), and developed a quick method of high specificity for the detection of complement‐bound HCV‐CIC. We found that there were higher frequencies of HCV‐C1q CIC than that of HCV‐factor B, and there was a deviation of complement from immunoglobulin (Ig) in HCV‐CIC. These findings suggest that immuno‐capture RT‐PCR (iRT‐PCR) for the detection of HCV‐bound CIC is a valuable method for the analysis of the composition of the immune complexes, and for the understanding of host immune response and immune pathogenesis in HCV‐infected individuals.