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Endomorphins 1 and 2 Inhibit IL‐10 and IL‐12 Production and Innate Immune Functions, and Potentiate NF‐κB DNA Binding in THP‐1 Differentiated to Macrophage‐Like Cells
Author(s) -
AZUMA Y.,
OHURA K.
Publication year - 2002
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2002.01128.x
Subject(s) - thp1 cell line , innate immune system , lipopolysaccharide , macrophage , microbiology and biotechnology , phagocytosis , biology , phorbol , cytokine , immune system , chemistry , immunology , cell culture , signal transduction , protein kinase c , biochemistry , in vitro , genetics
We evaluated immunological effects of opioid peptides endomorphins 1 and 2 on the production of interleukin‐10 (IL‐10) and IL‐12 cytokines, functions related to innate immunity and NF‐κB DNA binding in human cell line THP‐1. Endomorphins 1 and 2 inhibited lipopolysaccharide (LPS)‐stimulated IL‐10 and IL‐12 production in THP‐1 differentiated to macrophage‐like cells by phorbol 12‐myristate 13‐acetate (PMA). Similarly, they suppressed LPS‐stimulated IL‐10 and IL‐12 production in THP‐1 matured to monocytes by 1α,25‐dihydroxyvitamin D 3 . In addition, endomorphins 1 and 2 led to marked potentiation of NF‐κB binding in THP‐1 differentiated to macrophage‐like cells. Furthermore, these endomorphins further potentiated LPS‐induced NF‐κB binding. Moreover, they inhibited chemotaxis, phagocytosis of Escherichia coli and PMA‐stimulated production of hydrogen peroxide in THP‐1 differentiated to macrophage‐like cells. These results suggest that endomorphins 1 and 2 may inhibit THP‐1 functions, such as cytokine production and functions related to innate immune, and potentiate NF‐κB DNA binding in THP‐1.