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Dendritic Cells Present an Intracellular Viral Antigen Derived from Apoptotic Cells and Induce a T‐Cell Response
Author(s) -
VAN ZANTEN J.,
HOSPERS G. A. P.,
HARMSEN M. C.,
THE T. H.,
MULDER N. H.,
DE LEIJ L. F. M. H.
Publication year - 2002
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2002.01125.x
Subject(s) - microbiology and biotechnology , cytotoxic t cell , apoptosis , antigen presenting cell , antigen , immune system , cd8 , t cell , transfection , biology , dendritic cell , intracellular , cell culture , chemistry , immunology , in vitro , biochemistry , genetics
We investigated if dendritic cells (DCs) were able to present intracellularly located antigens derived from apoptotic cells to T cells, thereby inducing a CD4 + and a CD8 + response. A transfected cell line with the cytomegalovirus‐derived protein pp65 was triggered to go into apoptosis by ultraviolet B (UVB) irradiation, and after the uptake of apoptotic cells by DC, the activation and proliferation of T cells were determined. We found that DC efficiently phagocytosed apoptotic cells and induced a CD4 + and a CD8 + T‐cell response specific for the viral protein pp65. This mechanism can be useful for vaccination studies to induce an antiviral immune response.