Premium
CD14 + CD16 + Monocytes in the Course of Sepsis in Neonates and Small Children: Monitoring and Functional Studies
Author(s) -
Skrzeczyñska J.,
Kobylarz K.,
Hartwich Z.,
Zembala M.,
Pryjma J.
Publication year - 2002
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2002.01092.x
Subject(s) - cd14 , cd16 , sepsis , monocyte , immunology , medicine , antigen , flow cytometry , cd3 , cd8
The phenotype and function of peripheral blood monocytes change after trauma and during sepsis. The aim of the study was to evaluate monocyte expression of human leucocyte antigen (HLA)‐DR and Fc receptor III (FcR III) (CD16) in neonates and small children with high risk of sepsis (hospitalized at the intensive care unit). The reduced proportion of CD14 + HLA‐DR + monocytes was observed in all patients at the intensive care unit, while the increase of CD16 expression on monocytes was observed in the course of sepsis. The measurement of CD16 expression on monocytes also proved to be more useful for monitoring patient. The proportion of both CD14 dim CD16 + and CD14 high CD16 + monocytes increased during sepsis; however, monocytes showed reduced ability to phagocytose Escherichia coli , compromised ability to cooperate with T cells and reduced CD86 expression in parallel to HLA‐DR depression. The reduced interleukin (IL)‐1 but rather increased IL‐10 production was associated with sepsis. The differences between CD14 + CD16 + monocytes of healthy donors and patients with sepsis are discussed.