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Decreased Urokinase Receptor Expression on Granulocytes in HIV‐Infected Patients
Author(s) -
Storgaard M.,
Obel N.,
Black F. T.,
Møller B. K.
Publication year - 2002
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2002.01062.x
Subject(s) - urokinase receptor , downregulation and upregulation , flow cytometry , receptor , receptor expression , biology , supar , chemotaxis , plasminogen activator , granulocyte , cd18 , microbiology and biotechnology , immunology , integrin alpha m , chemistry , endocrinology , biochemistry , gene
Pericellular proteolysis initiated by receptor‐bound urokinase‐type plasminogen activator (uPA) is considered important for directed migration of granulocytes to inflammatory sites. Using flow cytometry and whole‐cell binding of radiolabelled‐uPA, we found a high level of uPA‐receptor (uPAR) expression in granulocytes (3.9 × 104 ± 0.9 × 104 sites/cell). Modulation of uPAR expression was assessed in the presence of chemoattractant gradients. Our findings demonstrate that interleukin (IL)‐8, leukotriene B 4 (LTB 4 ) and formyl‐methionyl‐leucyl‐phenylalanine (f MLP) caused a dose‐dependent upregulation of uPAR on granulocytes in healthy controls. Modulation of uPAR expression is known to regulate chemotactic response. As determined by flow cytometry, uPAR expression by granulocytes from human immunodeficiency virus (HIV)‐infected patients was distinctly lower than that of healthy control cells ( P < 0.001). However, upregulation of uPAR in response to chemoattractants was similar to that observed in healthy controls. In HIV‐infected patients, the uPAR expression on granulocytes correlated ( P < 0.001, n = 10) with the number of CD4 + blood cells. In contrast, the expression of IL‐8 receptor, CD11b, CD18 and CD62 was not significantly altered in HIV‐patients compared with healthy controls.