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Abstracts ‐ Tuesday 24th July 2001
Author(s) -
Lu, L,
Osmond, D.G.
Publication year - 2001
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2001.0540s1002.x
Subject(s) - citation , library science , information retrieval , computer science
This FREE journal suppl. entitled: 11th International Congress of Immunology Stockholm, Sweden 22-27th July 2001Abstracts - Tuesday 24th July 2001: no. C4.Tue.1.6/138Osteopetrotic (op/op) mice are deficient in macrophages and osteoclasts due to a CSF-1 gene mutation. This study aimed to evaluate the effect of these deficiencies and of CSF-1-dependent mechanisms on B lymphopoiesis in bone marrow. B cell development and apoptoses have been examined in bone marrow of op/op mice, using immuno-fluorescence labeling and flow cytometry. Bone marrow cellularity was greatly reduced in op/op mice compared with normal littermates. However, precursor B cells were disproportionately decreased, most markedly at the pre-B cell stage. Precursor B cells displayed elevated apoptotic incidences both ex vivo and in short-term culture. Addition of recombinant CSF-1 reduced the incidence of apoptosis among precursor B cells in short-term cultures of whole bone marrow suspensions from normal mice but not in cultures of sorted B lineage cells. The findings provide evidence that CSF-1-dependent mechanisms can strongly influence the survival of precursor B cells in mouse bone marrow. It is proposed that the local or systemic levels of CSF-1 during ontogeny may thus play a role in regulating B cell production within the bone marrow microenvironment.link_to_OA_fulltex