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Experimental IgG Antibody Production In vitro by Peripheral Blood and Tonsil Surface γ + B Lymphocytes from Plasmodium falciparum ‐Immune West Africans
Author(s) -
Garraud O.,
Diouf A.,
Nguer C. M.,
Tall A.,
Marrama L.,
Perraut R.
Publication year - 2001
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2001.01011.x
Subject(s) - peripheral blood mononuclear cell , antigen , tonsil , antibody , immune system , immunology , biology , plasmodium falciparum , cd19 , in vitro , virology , microbiology and biotechnology , malaria , biochemistry
Antigen reactive B cells in tonsil specimens from teenagers from a region moderately exposed to P . falciparum were capable of being differentiated in vitro and producing specific immunoglobulin (Ig)G in up to 33% of individual experiments. Mononuclear cells or purified sγ + CD19 + B cells from peripheral blood or tonsil specimens from P. falciparum ‐immune Senegalese subjects produced antigen‐specific IgG upon appropriate stimulation in vitro . One fraction of this IgG was produced de novo by differentiated B cells and another fraction was likely bound on the surface of circulating or resident CD19 + sγ + B cells which were found in significantly greater numbers in individuals from rural Senegal as compared to nonimmune European controls. This study further documents the baseline levels of in vitro driven anti‐ P. falciparum IgG antibody production by mononuclear cells from blood and tonsils in immune populations exposed to P. falciparum differentially. Furthermore, this study demonstrates the relevance and potential utility of tonsils as a source of B lymphocytes to characterize further specific antibody responses to P. falciparum antigens in immune populations.