Premium
Blood γδ T Cells and γδ TCR V Gene Specificities in a Single Missense Mutation (L→Q271) in the Common Gamma Chain Gene
Author(s) -
Schmalstieg F. C.,
Palkowetz K. H.,
Rudloff H. E.,
Goldman A. S.
Publication year - 2001
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2001.01007.x
Subject(s) - t cell receptor , missense mutation , cd8 , cd3 , biology , microbiology and biotechnology , mutation , t cell , cytotoxic t cell , gene , immunology , genetics , antigen , immune system , in vitro
The numbers of blood CD4 + , CD8 + , and CD4 − CD8 − T cells bearing αβ T‐cell receptor (TCR) or γδ TCR molecules in males with a single missense mutation (L→Q271) in the common gamma chain gene (γ c ) were investigated by flow cytometry. Virtually all XCID L→Q271 blood T cells that were CD4 + or CD8 + displayed αβ TCR but no γδ TCR. In contrast, CD4 − CD8 − T cells from affected males usually displayed γδ TCR, but no αβ TCR. The γδ TCR specificities were also studied. Except for the oldest subject, there was a direct relationship between blood CD3 + T cells that displayed γδ TCR and Vγ9 and Vδ2a specificities. Relative frequencies of CD3 + blood T cells that were Vγ9 + or Vδ2a + were inversely related to age. In the oldest patient, the only detected γδ TCR specificity was Vδ1. Thus, in contrast to mice with no γ c , XCID L→Q271 blood T cells that bear γδ TCR with Vγ9/Vδ2a specificities develop but then decline in late childhood and thereafter. TCR with the Vδ1 specificity then appear in older survivors with XCID L→Q271 .