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Cytokine Gene Expression in Healing and Non‐Healing Cases of Cutaneous Leishmaniasis in Response to In vitro Stimulation with Recombinant gp63 Using Semi‐Quantitative RT–PCR
Author(s) -
Habibi G. R.,
Khamesipour A.,
McMaster W. R.,
Mahboudi F.
Publication year - 2001
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2001.00990.x
Subject(s) - peripheral blood mononuclear cell , immunogen , cytokine , immunology , reverse transcription polymerase chain reaction , interferon gamma , stimulation , recombinant dna , leishmania infantum , interleukin 4 , cutaneous leishmaniasis , gene expression , medicine , interleukin , leishmaniasis , in vitro , biology , gene , visceral leishmaniasis , antibody , biochemistry , monoclonal antibody
Objectives of this study were to test the cytokine gene expression in peripheral blood mononuclear cells (PBMCs) from cases with nonhealing and healing cutaneous leishmaniasis (CL) in response to in vitro stimulation of recombinant gp63 (rgp63) and soluble Leishmania antigen (SLA). Healing and nonhealing cases are, respectively, defined as recovered from disease and refractory to various treatments. To evaluate the type of immunological response, mRNA transcription level for interleukin (IL)‐4, IL‐10, IL‐12 and interferon (IFN)‐γ were determined using semiquantitative reverse transcription–polymerase chain reaction (RT–PCR) technique in PBMCs of these volunteers. The results clearly demonstrated a high level of IL‐4 expression in nonhealing cases of CL and a low expression level of transcripts for IFN‐γ and IL‐12. In contrast, a high level of IFN‐γ and IL‐12 expression and a low level of IL‐4 and IL‐10 expression were detected in the healing cases. These findings not only support the balance of Th1/Th2 cytokines in the inducing predominant profile in healing and nonhealing cases, but it may also show the potential of rgp63 as a proper immunogen which might induce protective responses.