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Regulation of Surface and Intracellular Expression of CTLA‐4 on Human Peripheral T Cells
Author(s) -
Wang X.B.,
Zheng C.Y.,
Giscombe R.,
Lefvert A. K.
Publication year - 2001
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2001.00985.x
Subject(s) - ctla 4 , cytotoxic t cell , il 2 receptor , intracellular , microbiology and biotechnology , biology , cd8 , cd28 , antigen , t cell , interferon gamma , downregulation and upregulation , interleukin 21 , chemistry , immune system , immunology , in vitro , gene , biochemistry
Cytotoxic T‐lymphocyte‐associated antigen (CTLA‐4) is an important downregulator of T‐cell activation. In order to analyze the expression and regulation of CTLA‐4 on human peripheral T cells, CTLA‐4 mRNA and protein expression were determined using analysis by reverse transcription–polymerase chain reaction (RT–PCR) and FACs, respectively. Intracellular CTLA‐4 was constitutively expressed in unstimulated CD4 + and CD8 + T cells. Interleukin (IL)‐2 induced a dose‐dependent increase of both intracellular and surface expression of CTLA‐4 (CD152). Most of the CD4 + and CD8 + cells expressing CTLA‐4 also expressed CD25. Interferon (IFN)‐γ induced the upregulation of CTLA‐4 expression via antigen‐presenting cells (APC) activation. The CTLA‐4delTM mRNA (550 bp) had a shorter half‐life than the full length CTLA‐4 mRNA and the expression was downregulated upon activation of the cells by treatment with IL‐2. Given an inhibitory role of CTLA‐4 and CD4 + CD25 + T cells in immune responses, the present findings suggest that IL‐2‐induced immunosuppression may result from its stimulatory effect of the CTLA‐4 expression.