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The Effect of In vivo Depletion of NKR‐P1 + or CD8 + Lymphocytes on the Acute Rejection of Allogeneic Lymphocytes (ALC) in the Rat
Author(s) -
Løvik G.,
Vaage J. T.,
Rolstad B.,
Naper C.
Publication year - 2001
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2001.00979.x
Subject(s) - in vivo , ctl* , cd8 , cytotoxic t cell , in vitro , population , lymphocyte , immunology , cytotoxicity , biology , microbiology and biotechnology , natural killer cell , lymphokine activated killer cell , interleukin 21 , immune system , medicine , biochemistry , environmental health
We have depleted lymphocyte subsets in PVG and AO rats with MoAbs 3.2.3 (against NKR‐P1 on NK and NK/T cells) and OX‐8 (against CD8 on CTL and NK cells), and examined the effect on the killing of YAC‐1 target cells in vitro and the effect on the acute rejection of small allogeneic lymphocytes in vivo (allogeneic lymphocyte cytotoxicity, ALC). While 3.2.3 treatment led to only a partial depletion of 3.2.3‐positive cells in PVG rats, this treatment drastically reduced the number of NKR‐P1 + cells in AO rats, abolished splenic NK activity against the NK‐sensitive tumour target YAC‐1, and markedly diminished the ALC response. Rats treated with OX‐8 for 1 day showed a similar loss of NK cell function in vivo and in vitro. However, in rats treated with OX‐8 for 3 days a 3.2.3 + and OX‐8 − population consisting of NK cells appeared, restoring ALC. The results demonstrate that NK cell responses can be greatly diminished after in vivo treatment with these MoAbs. Furthermore, they demonstrate that ALC is not necessarily linked to expression of the CD8 molecule.