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Segregation of Effector Mechanisms in a Tumour‐Specific CD8 + T‐Cell Clone Correlates with CD30 Expression
Author(s) -
Sun Y.,
Song M.,
Maeurer M. J.,
Schadendorf D.
Publication year - 2001
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2001.00971.x
Subject(s) - ctl* , cytotoxic t cell , clone (java method) , biology , interleukin 2 , cd8 , effector , antigen , cytokine , interferon gamma , microbiology and biotechnology , cancer research , immunology , gene , biochemistry , in vitro
In this study we have analyzed CD30‐antigen expression in three melanoma‐directed cytotoxic T lymphocyte (CTL) clones with a T helper 0 (Th0)‐like cytokine secretion profile (i.e. interleukin (IL)‐4, IL‐5, and interferon (IFN)‐γ). We show that all CTL clones expressed high levels of CD30 upon contact with the autologous tumour cells. One CTL clone, termed A2 with a monoclonal feature was selected for further analyses and found its CD30 expression dependent on the presence of IL‐4. Functionally, a CD30‐expressing A2 CTL was capable of producing higher amounts of IFN‐γ (up to 1.5‐fold) and IL‐4 (up to two‐fold) than its CD30 − counterpart. Furthermore, CD30‐positive A2 CTL displayed an at least three‐fold greater proliferative response to the tumour cell stimulation, contrasting with CD30 − CTL. However, the antitumour cytotoxic activity of A2 CTL was not modulated by the CD30 expression. These results suggest that CD30 antigen can be inducible on a subset of tumour‐directed CD8 + CTL, and that this subset of cells may have profound effector functions, such as cytokine secretion, proliferation, and cytotoxicity.