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T‐Cell Enforced Invariance of the Antibody Repertoire in the Immune Response Against a Bacterial Carbohydrate Antigen
Author(s) -
Rademaekers A.,
Specht C.,
Kölsch E.
Publication year - 2001
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2001.00864.x
Subject(s) - idiotype , antigen , immune system , biology , antibody , cd40 , b 1 cell , t cell , immunology , b cell , antigen presenting cell , clone (java method) , microbiology and biotechnology , cytotoxic t cell , in vitro , monoclonal antibody , biochemistry , gene
The humoral response against the bacterial polysaccharide antigen α(1→3) dextran (Dex) is controlled by J558 idiotype‐(Id) specific T cells. These T cells of which the cell clone 178–4 Ts is a representative by all relevant criteria, recognize J558 Id‐bearing B cells in an I‐E d ‐restricted manner. Costimulation via CD28/B7‐1 but not via CD40/CD40L leads to T‐cell activation. These T cells do not only suppress B cells producing the immunoglobulin (Ig)G3 isotype but also support the survival and clonal expansion of J558 Id positive B cells both in vivo and in vitro . This T‐cell mediated dominance of the J558 idiotype limits the appearance of antibodies carrying other more diverse idiotypes which appear in immunized BALB/ c nu/nu mice where no regulatory T cells occur. This T‐cell mediated antibody invariance could be a strategy of the immune system responding to highly conserved antigens like polysaccharides, different from those against protein antigens, where diversity is assumed to be the basis for a successful response.