Premium
Distinct Amplification of the C5a‐Receptor Pathways in Normodense and Hypodense Eosinophils of Patients with Atopic Dermatitis
Author(s) -
Czech W.,
Dichmann S.,
Herouy Y.,
Rheinen H.,
Elsner J.,
Kapp A.,
Norgauer J.
Publication year - 2001
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2001.00860.x
Subject(s) - receptor , immunology , atopic dermatitis , reactive oxygen species , medicine , biology , microbiology and biotechnology
In patients with atopic dermatitis two different types of blood eosinophils with distinct density can be isolated. The normodense cells represent the huge majority in count, whereas the hypodense eosinophils are characterized by higher effector activity. To understand the altered functional responsiveness of these two cell subtypes, the expression of C5a receptors as well as C5a‐induced signal pathways and the production of reactive oxygen metabolites have been analyzed. Chemiluminescence measurements revealed significant higher production of reactive oxygen metabolites in hypodense eosinophils in comparison to normodense cells. However, no difference in the expression level of C5a receptors as well as in the C5a‐induced Ca 2+ ‐transients between normodense and hypodense eosinophils were found. In contrast, hypodense eosinophils showed a significantly higher actin polymerization response and phosphatidylinositol 4,5 bisphosphate 3‐kinase activation after stimulation with C5a than normodense eosinophils. Therefore, normodense and hypodense eosinophils from the blood of patients with atopic dermatitis are characterized by differential amplification of C5a‐receptor signal pathways, which might explain the differences in their proinflammatory activity.