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Human Autologous Monocytes and Monocyte‐Derived Macrophages in Co‐Culture with Carcinoma F‐Spheroids Secrete IL‐6 by aNon‐CD14‐Dependent Pathway
Author(s) -
Heimdal J.H.,
Aarstad H. J.,
Olsnes C.,
Olofsson J.
Publication year - 2001
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2001.00853.x
Subject(s) - cd14 , secretion , monocyte , spheroid , tumor necrosis factor alpha , lipopolysaccharide , stimulation , cytokine , microbiology and biotechnology , cell culture , chemistry , cancer research , immunology , biology , medicine , endocrinology , flow cytometry , genetics
The secretion of interleukin (IL)‐1β, IL‐6 and tumour necrosis factor (TNF)‐α were compared when freshly isolated autologous monocytes or monocytederived macrophages (MDMs) were co‐cultured in vitro with autologous fragment (F)‐spheroids established from a series of head and neck squamous cell carcinoma (HNSCC) patients. F‐spheroids were generated from the malignant tumour (M‐spheroids) or from benign mucosa (B‐spheroids) from which the tumour originated control. If monocytes maturated towards MDMs before co‐culture, the IL‐6 secretion declined dependent on the extent of the MDM maturation by both M‐ and B‐spheroid stimulation. When MDMs maturated in continuous co‐culture, a steady‐state secretion of IL‐6 continued for several days but diminished when the culture medium was changed every 24 h. No co‐culture‐induced IL‐1β or TNF‐α was determined. Both the cytokine secretion and the mRNA gene expression revealed a different monocyte/MDM activation when co‐culture and lipopolysaccharide (LPS)‐stimulation were compared. Addition of anti‐CD14 (10 µg/ml) decreased monocyte LPS‐stimulated, but increased monocyte co‐culture stimulated IL‐6 secretion. In conclusion, M‐ and B‐spheroids similarly stimulated monocytes and to a lesser extent MDMs. MDMs that maturated with F‐spheroids present, retained responsiveness at the monocyte level. Co‐culture‐induced monocyte stimulation, as measured by IL‐6 secretion, was not dependent on activation via the CD14 molecule.