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B‐Cell Depletion Fails to Abrogate the Induction of Oral Tolerance of Specific Th1 Immune Responses in Mice
Author(s) -
Peng Peng,
Chang Chang,
Myeong Soo Lee,
C.-C. Jay Kuo
Publication year - 2000
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2000.00700.x
Subject(s) - ovalbumin , spleen , immunology , immune system , antigen , adoptive cell transfer , immune tolerance , t cell , b cell , antibody , adjuvant , biology , egg white , medicine , food science
Antigen presentation by resting B cells has been shown to induce peripheral tolerance to intravenous (i.v.) administered soluble protein antigens. We further examined the role of resting B cells in the induction of oral tolerance. Mice were treated continuously from birth with rabbit antimouse IgM serum for 5 weeks. Immunohistological studies revealed that anti‐IgM treatment depleted B cell‐aggregated follicles in intestinal Peyer's patches. At 4‐weeks‐old, B cell‐depleted mice were fed 25 mg ovalbumin or given 10% chicken egg white to drink for 5 days. Anti‐IgM treatment was stopped 2 days after the last feed. Ten weeks later, the mice were immunized with 100 μg ovalbumin emulsified with complete Frund's adjuvant. Their T helper 1 (Th1) cell‐regulated systemic delayed‐type hypersensitivity, IgG2a antibody responses and spleen cell production of interferon‐r and interleukin‐2 were suppressed by prior ovalbumin or egg white feeding during anti‐IgM treatment. Active suppression of Th1 immune responses was also demonstrated following adoptive transfer of egg white‐fed donor spleen cells collected during anti‐IgM treatment to naïve recipients. Although enormous small resting B cells are aggregated in the mantle zones of follicles of intestinal Peyer's patches, they are not the antigen‐presenting cells seen in the induction of oral tolerance.