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Augmented Expression of Daintain/Allograft Inflammatory Factor‐1 is Associated with Clinical Disease: Dynamics of Daintain/Allograft Inflammatory Factor‐1 Expression in Spleen, Peripheral Nerves and Sera during Experimental Autoimmune Neuritis
Author(s) -
Мikhail Pashenkov,
Efendic,
Wanxue Zhu,
Zou Zou,
Östenson,
Mustafa
Publication year - 2000
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2000.00682.x
Subject(s) - medicine , neuritis , immunology , proinflammatory cytokine , spleen , pathogenesis , autoimmune disease , inflammation , pathology , antibody , psychiatry
Experimental autoimmune neuritis (EAN) is an animal model of Guillain–Barré syndrome, characterized by inflammation and demyelination of the peripheral nervous system (PNS). Daintain/allograft inflammatory factor‐1 (daintain/AIF‐1) is a novel interferon‐γ–inducible protein expressed by macrophages during organ specific autoimmune diseases. To study the involvement of daintain/AIF‐1 in EAN we induced EAN in Lewis rats by immunizing with bovine PNS myelin (BPM) and complete Freund's adjuvant (CFA). The expression of daintain/AIF‐1 was examined in the spleen, peripheral nerves and sera during the course of EAN by immunohistochemistry and radioimunoassay (RIA). The expression of daintain/AIF‐1 in the spleen and in the sciatic nerves peaked at the preclinical stage (day 7 post immunization (p.i.)) and at the height (day 15 p.i.) of clinical EAN, consistent with a disease promoting role for daintain/AIF‐1. Daintain/AIF‐1 expressing cells represented a subset of ED1 + or CD11b/c + mononuclear cells. A significant increase of daintain/AIF‐1–like immunoreactivity in sera occurred at the preclinical stage of EAN. Taken together, these data indicate that daintain/AIF‐1 may play a proinflammatory role in the pathogenesis of EAN.