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Apoptotic Cells Actively Inhibit the Expression of CD69 on Con A Activated T Lymphocytes
Author(s) -
Erwei Sun,
L. Zhang,
Yingjian Zeng,
Qing Ge,
Ming Zhao,
Wei Gao
Publication year - 2000
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2000.00666.x
Subject(s) - apoptosis , cytotoxic t cell , microbiology and biotechnology , t cell , biology , cycloheximide , cd8 , cd3 , k562 cells , programmed cell death , cell culture , chemistry , antigen , immunology , immune system , biochemistry , in vitro , genetics , protein biosynthesis
Although apoptosis is commonly viewed as a silent cell death without damage to adjacent tissues, the effect of apoptosis on immunity has been unclear. We have investigated the influence of apoptotic cells on T‐cell activation. The K562 or HL‐60 human leukemia cell lines that had been induced apoptosis by FTY720 or cycloheximide (CHX) were added into the culture of mouse spleen cells stimulated with Con A. Six to 20 h later, the expression of CD69, an early T‐cell activation antigen, was detected using flowcytometry. Living cells and necrotic cells served as control groups. Apoptotic K562 or HL‐60 cells induced by either FTY720 or CHX unanimously inhibited CD69 expression on the CD3 + mouse T cells while living and necrotic cells did not. The inhibition was proportional to the number of apoptotic cells and was different in the T‐cell subsets, showing a rapid and transient inhibition on the CD3 + CD8 + T‐cell activation but with a slow and continuous inhibition on CD3 + CD8 − T‐cell activation. In conclusion, the apoptotic cells actively inhibit a T‐cell activation that is independent of the cell lines or the apoptotic inducers, indicating that the apoptotic cells dominantly regulate T‐cell immunity.