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Flt‐3 Ligand, in Combination with Bovine Granulocyte–Macrophage Colony‐Stimulating Factor and Interleukin‐4, Promotes the Growth of Bovine Bone Marrow Derived Dendritic Cells
Author(s) -
Jayne Hope,
Dirk Werling,
Robert A. Collins,
Bea Mertens,
Chris Howard
Publication year - 2000
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.2000.00646.x
Subject(s) - follicular dendritic cells , dendritic cell , stem cell factor , bone marrow , biology , major histocompatibility complex , microbiology and biotechnology , mhc class ii , granulocyte macrophage colony stimulating factor , myeloid , interleukin 3 , antigen presenting cell , antigen presentation , immunology , stem cell , t cell , antigen , cytokine , haematopoiesis , immune system
Culture of bone marrow precursor cells with cytokines, including granulocyte–macrophage colony‐stimulating factor (GM–CSF), interleukin‐4 (IL‐4) and the tyrosine kinase receptor binding proteins Flt‐3 ligand (Flt‐3L) and stem‐cell factor (SCF), has previously been shown, in both mouse and human, to result in the generation of large numbers of dendritic cells. We extend these findings to bovine dendritic cells. Culture of bovine bone marrow cells with GM–CSF, IL‐4 and either Flt‐3L or SCF enhanced the generation of low buoyant‐density dendritic cells. However, only the addition of Flt‐3L to cells cultured with GM–CSF and IL‐4 was shown to increase the number of dendritic cells and induce the differentiation of dendritic cells with potent capacity to stimulate allogeneic T cells and resting CD4 + memory T cells. The effective ability to stimulate T cells was associated with the expression of major histocompatibility complex (MHC) class II molecules and CD80/86 by dendritic cells. Bovine bone marrow derived dendritic cells appeared to be exclusively of myeloid origin because they expressed the myeloid‐related antigens CD14, MyD‐1 and CD11b.