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Collagen‐Bound α 1 ‐Microglobulin in Normal and Healed Tissues and its Effect on Immunocompetent Cells
Author(s) -
Matteo Santin,
M. Cannas
Publication year - 1999
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1999.00597.x
Subject(s) - beta 2 microglobulin , scars , extracellular matrix , western blot , chemistry , immunohistochemistry , in vitro , pathology , matrix (chemical analysis) , collagen fibril , microbiology and biotechnology , anatomy , medicine , biology , immunology , biochemistry , chromatography , gene
The mechanisms devoted to the protection of the extracellular matrix collagen from the inflammatory insult are not fully understood. We investigated the presence of the immunosuppressive glycoprotein α 1 ‐microglobulin in healed tissues such as scars and periprosthetic membranes, comparing them with normal skin. Immunohistochemistry showed that α 1 ‐microglobulin was mainly present along collagen fibrils and in the epidermis. The presence of this protein was confirmed by Western blot of the tissue homogenates, while ELISA showed lower levels in the healed tissues. In vitro , the purified α 1 ‐microglobulin bound collagen by a cooperative mechanism and attenuated the collagen‐induced activation of the mononuclear cells.