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Costimulation of CD28 − T Cells Through CD3 and β 1 ‐Integrins Induces a Limited Th1 Cytokine Response
Author(s) -
Jussi Saukkonen,
A Tantri,
Jeffrey S. Berman
Publication year - 1999
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1999.00562.x
Subject(s) - cd28 , cytokine , cd3 , integrin , chemistry , immunology , microbiology and biotechnology , t cell , biology , immune system , cell , cd8 , biochemistry
The costimulatory molecule CD28 regulates antigen‐specific T‐cell proliferation and the synthesis of multiple cytokines. The absence of CD28 on a subset of CD8 bright+ T cells suggests that these cells may utilize alternative costimulatory pathways or have a limited cytokine response to presented antigen. We used fibronectin, a ligand for the β 1 ‐integrins α 4 β 1 and α 5 β 1 , as an alternate costimulatory ligand to assess the functional phenotype of CD8 bright+ CD28 − T cells. CD25 expression was significantly up‐regulated in CD8 bright+ CD28 − T cells by immobilized anti‐CD3 i with fibronectin. Costimulation with fibronectin also significantly augmented anti‐CD3 i ‐induced IFN‐γ production only among CD8 bright+ CD28 − T cells. The CD8 bright+ CD28 − T cells did not produce significant IL‐2 and IL‐10 even in response to maximal stimulation with phorbol myristate acetate and ionomycin. These data support a costimulatory role for ß 1 ‐integrins in CD8 bright+ CD28 − T cells and indicate that CD8 bright+ CD28 − T cells have a restricted Th1 cytokine repertoire.