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Biological Properties of Interferon‐α Produced Ex Vivo by Whole Blood of Patients Infected by Human Immunodeficiency Virus‐1
Author(s) -
Wassim Chehadeh,
Didier Hober,
Vincent Chieux,
G. Alm,
Jack Harvey,
Georges Lion,
Y Mouton,
P Wattré
Publication year - 1999
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1999.00552.x
Subject(s) - vesicular stomatitis virus , sendai virus , virology , virus , interferon , in vitro , biology , in vivo , ex vivo , whole blood , cytopathic effect , immunology , intracellular , biochemistry , microbiology and biotechnology
We investigated the biological properties of interferon (IFN)‐α produced by Sendai virus (SV)‐activated whole blood cultures in 20 patients infected with human immunodeficiency virus (HIV)‐1 and 24 healthy controls. Supernatants of cultures were assayed for IFN‐α by using an immunological method (DELFIA), biological methods and an in‐vitro MxA induction assay. The levels of intracellular MxA protein were detected by an immunochemiluminescence assay. The levels of IFN‐α in patients measured by DELFIA were significantly lower than those in healthy controls ( P < 0.0001), but the antiviral activity of IFN‐α in patients infected with HIV‐1 was lower than predicted from DELFIA. The IFN‐α produced by cells of patients infected with HIV‐1 was able to induce MxA protein in human amnions WISH cells but was unable to protect these cells against Vesicular Stomatitis Virus (VSV)‐induced cytopathic effects. A relative increased capability to induce the production of MxA protein in vitro was observed with the IFN‐α contained in culture supernatant of virus‐activated whole blood of HIV‐1‐infected patients with increased levels of MxA in their peripheral blood. These data suggest that biological properties of IFN‐α produced in the course of HIV‐1 infection are different from those observed with IFN‐α of healthy subjects.