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Characterization of the Three Immunoglobulin G Subclasses of Macaques
Author(s) -
Patrick Calvas,
Pôl-André Apoil,
Françoise Fortenfant,
F. Roubinet,
Jennifer S. Andris,
Daniela Capra,
Antoine Blancher
Publication year - 1999
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1999.00540.x
Subject(s) - complementary dna , biology , rhesus macaque , subclass , microbiology and biotechnology , gene , macaque , antibody , coding region , genomic dna , primate , southern blot , genetics , paleontology , neuroscience
Southern blot experiments with genomic DNA samples of rhesus monkeys and crab‐eating macaques and human C gamma‐specific probes indicated that the two macaque species studied here possessed three C gamma genes per haploid genome. By amplifying the cDNA from macaque–mouse hybridomas, the coding sequences of two different rhesus monkey immunoglobulin (Ig)G subclasses, IgG1 rh (Cγ1 rh ) and IgG2 rh (Cγ2 rh ), and one crab‐eating macaque IgG subclass IgG1 mafa (Cγ1 mafa ), were characterized. None of the 16 rhesus monkey–mouse hybridomas studied here secreted IgG of the third subclass IgG3 rh (Cγ3 rh ). The Cγ3 rh gene was partly characterized at the genomic level. The cDNA of the Cγ3 rh gene was amplified from mRNA of rhesus monkey peripheral blood mononuclear cells (PBMC). The results are analysed in terms of phylogenesis of the C gamma genes. The cDNA sequences coding for the Cμ and the Cκ domains of rhesus monkey Ig were established and compared to their human and non‐human primate counterparts.