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Selection of Distinct Vα/β T‐Cell Receptor Families During In Vivo and In Vitro T‐Cell Maturation
Author(s) -
Nadia Caccamo,
Guido Sireci,
Juraj Iványi,
Alfredo Salerno,
Francesco Dieli
Publication year - 1999
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1999.00527.x
Subject(s) - t cell receptor , in vitro , biology , microbiology and biotechnology , transgene , in vivo , concanavalin a , t cell , immune system , lymph node , peptide , genetically modified mouse , immunology , gene , genetics , biochemistry
The experimental conditions influencing the use of Vαβ TCR families were examined in lymph node (LN) cells from peptide‐immunized C57BL/6 and Vβ8.2 transgenic mice. Expanded proportions of Vβ5, Vβ8.2, Vβ9, Vβ12 and Vβ14 positive cells and an association of Vβ8.2 with Vα11 was found in freshly harvested 8‐day or 34‐day immune LN cells. In contrast, peptide‐specific T‐cell lines generated in vitro from 8‐day immune lymph node cells were found to be almost exclusively of the Vα2/Vβ12 family. However, T‐cell lines originating from Vβ8.2 transgenic mice did not show preferential Vα usage. Anti‐Vβ8.2 antibody produced different effects: when added to cultures of LN cells from C57BL/6 or Vβ8.2 transgenic strains, the peptide‐induced proliferation was suppressed; however, following the injection of mice, subsequent in vitro proliferation and cytokine production induced by both peptide and Concanavalin A was suppressed in Vβ8.2 transgenic, but much less in C57BL/6 mice. Hence, compensatory expansion of different Vβ gene products occurred in vivo , but not under the employed in vitro conditions. In conclusion, these results suggest that the TCR family usage is influenced by the experimental conditions in which the T cells are selected and expanded and by the genetic potentials of the precursor pool.