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Preparation of IgG Subclass Allotypes from Polyclonal IgG
Author(s) -
Oxelius Va
Publication year - 1999
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1046/j.1365-3083.1999.00521.x
Subject(s) - allotype , subclass , polyclonal antibodies , antibody , chemistry , microbiology and biotechnology , immunoglobulin g , immunology , biology
Two allotypes have been identified for each of the IgG subclasses IgG1, IgG2 and IgG3. These allotypes are referred to as G1m(a) and G1m(f), G2m(n) and G2m(‐n), and G3m(g) and G3m(b). Using a pool of normal human serum and a combination of preparative electrophoresis, DEAE ion‐exchange and protein A–Sepharose chromatography, it was possible to separate G1m(f) from G1m(a), G2m(‐n) from G2m(n) and G3m(g) from G3m(b). Purification of G2m(‐n) molecules is of special interest as no genetic marker has been found to identify this allotype.